Part:BBa_K861173:Experience

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Applications of BBa_K861173

Our experiements was performed as bellow:
The correct clones were cultured in 96-well plate at 37℃ for 24 hours,then the fluorescence and absorbance at 600 nm were recorded on a SpectraMax M2 plate reader.All fluorescence was normalized with absorbance at 600 nm.The cell density was characterized by the absorbance at 600nm,which increased with glucose concentration.The result is showed in the figure bellow.

In this figure, the fluorescence of RFP generator promoted by PcstA decreased when concentration of glucose rose,that is to say,promoter PcstA can be activated by CRP at low glucose concentration.

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